Meat Tenderness Genetic and Genomic Variation Sources in Commercial Beef Cattle

Published in: Journal of Food Quality

1. María Salud Rubio Lozano,
2. Selene Alfaro-Zavala, 
3. Ana María Sifuentes-Rincón,
4. Gaspar Manuel Parra-Bracamonte, et al.

To assess genetic group (GGR; Bos indicus [Bi], Bos taurus [Bt] and crosses [BtxBi]) and to confirm the calpain (CAPN316 and CAPN4751) and calpastatin (CAST-T1) single nucleotide polymorphism (SNP) effects on Warner–Bratzler shear force (WBSF), 196 animals under commercial conditions were analyzed. A model was fitted including the effect of GGR and considering residuals as adjusted WBSF (aWBSF). Another model was fitted to evaluate the SNP effect on aWBSF. Allele substitution effect (α) and combined favorable alleles in CAPN and CAST on aWBSF were evaluated. GGR showed a significant effect (P < 0.0001) on WBSF; Bt and BtxBi had the lower WBSF. CAPN316 showed significant effect on aWBSF with an α = −0.549 kg. The combined effect of CAPN and CAST showed significant (P < 0.0056) reduction on aWBSF of 0.239 kg by favorable allele. The results remarked the importance of GGR and confirmed CAPN316 and combined effect of CAPN and CAST on prediction of meat tenderness.

Knowledge of main factors related to tenderness, as a key related factor to beef consumer satisfaction, would serve as a resource for commercial beef classification and management under slaughterhouse conditions. Confirmation on combined marker effects provides strong validation for marker-assisted management use of these technologies under industry conditions. Additionally, the presented results may be used as a reference for research in the beef industry aimed to provide improvement on beef tenderness to the final product.

http://onlinelibrary.wiley.com/doi/10.1111/jfq.12185/abstract

Markers association with Mexican bovine meat quality traits

Association of CAPN1 316, CAPN1 4751 and TG5 markers with Mexican bovine meat quality traits

Bonilla C A
Rubio M S
Sifuentes A M
Parra-Bracamonte G M
Arellano V W
Méndez M RD
Berruecos J M
Ortiz R

Summary


The aim of this study was to determine allele and genotype frequencies for CAPN1 316, CAPN1 4751 and TG5 molecular markers, and to assess associations with Mexican beef quality traits. Longissimus dorsi samples were collected from North, Central and Southern Mexico. They were typified by CAPN1 316 and CAPN1 4751 using the allelic discrimination assay and the TG5 marker was typed by PCR-RFLP. Meat quality traits included intramuscular fat content (IMF) and tenderness evaluation determined by Warner-Bratzler shear force (WBSF) at 24 hrs postmortem. The association test was achieved using a mixed model, including genotypes, genetic group (GG) and sampling location as fixed effects. Least squares means and significant interactions (P <0.05) were compared with the method of least significant difference by the Mixed procedure. Favorable genotype CAPN1 316 CC has a low frequency (0.03). Genotype frequencies for CAPN1 4751 were homogeneous for favorable (CC) and unfavorable (TT) genotypes (0.26 and 0.28, respectively). The TG5 CC genotype had a frequency of 0.73, while the TT genotype was 0.01. The means for WBSF and IMF were 4.08 ± 1.35kg and 5.23 ± 2.14%, respectively. For WBSF, the effect of the sampling site and the CAPN1 316 genotypes were significant (P <0.05). Samples collected from Hermosillo, Sonora, had the lowest WBSF (P <0.05), while those collected in Veracruz were tougher (WBSF = 5.267 kg). For IMF, the effect of GG and TG5 genotypes were significant (P <0.05). CAPN1 316 and TG5 markers have a significant effect on certain beef quality traits and thus allow Mexican beef characterization.


Soon in: http://www.funpecrp.com.br/gmr2008b/issue.asp