Markers association with Mexican bovine meat quality traits

Association of CAPN1 316, CAPN1 4751 and TG5 markers with Mexican bovine meat quality traits

Bonilla C A
Rubio M S
Sifuentes A M
Parra-Bracamonte G M
Arellano V W
Méndez M RD
Berruecos J M
Ortiz R

Summary


The aim of this study was to determine allele and genotype frequencies for CAPN1 316, CAPN1 4751 and TG5 molecular markers, and to assess associations with Mexican beef quality traits. Longissimus dorsi samples were collected from North, Central and Southern Mexico. They were typified by CAPN1 316 and CAPN1 4751 using the allelic discrimination assay and the TG5 marker was typed by PCR-RFLP. Meat quality traits included intramuscular fat content (IMF) and tenderness evaluation determined by Warner-Bratzler shear force (WBSF) at 24 hrs postmortem. The association test was achieved using a mixed model, including genotypes, genetic group (GG) and sampling location as fixed effects. Least squares means and significant interactions (P <0.05) were compared with the method of least significant difference by the Mixed procedure. Favorable genotype CAPN1 316 CC has a low frequency (0.03). Genotype frequencies for CAPN1 4751 were homogeneous for favorable (CC) and unfavorable (TT) genotypes (0.26 and 0.28, respectively). The TG5 CC genotype had a frequency of 0.73, while the TT genotype was 0.01. The means for WBSF and IMF were 4.08 ± 1.35kg and 5.23 ± 2.14%, respectively. For WBSF, the effect of the sampling site and the CAPN1 316 genotypes were significant (P <0.05). Samples collected from Hermosillo, Sonora, had the lowest WBSF (P <0.05), while those collected in Veracruz were tougher (WBSF = 5.267 kg). For IMF, the effect of GG and TG5 genotypes were significant (P <0.05). CAPN1 316 and TG5 markers have a significant effect on certain beef quality traits and thus allow Mexican beef characterization.


Soon in: http://www.funpecrp.com.br/gmr2008b/issue.asp